In this study, there are certain limitations, including (1) the detailed mechanisms explaining how PARD3B is involved in proliferation and colony formation of human glioblastoma cells should be further investigated; (2) because both U87 MG and GBM8401 cell lines were derived from female GBM patients, the cells from male patients also must be checked; and (3) animal studies should be carried out to confirm our present findings. This evidence concerns the gene PARD3B and glioblastoma.