ACTB and sporotrichosis: Based on our experience with the diagnosis of sporotrichosis, the suitability of DNA samples for the PCR-based assays should be evaluated by amplifying universal markers such as the ITS1/2+5.8S region for fungal DNA obtained from pure cultures [96]; GAPDH gene (chr12) for clinical samples containing human DNA (i.e., fresh or formalin-fixed and paraffin-embedded tissue blocks) [97]; 28S region for clinical samples containing cat DNA [98]; and the β-actin gene for samples containing murine DNA [99].