Another in vitro study observed significant a synergistic induction of apoptosis in the co-treatment of butyrate with tumour necrosis factor-alpha (TNF- α), anti-Fas agonist antibody, or TNF-related apoptosis-inducing ligand (TRAIL) against the MCF7, T47-D, and BT-20 breast cancer cell lines [28]. The gene discussed is TNFSF10; the disease is breast carcinoma.