It is possible that (1) the variants analyzed in this study do not play a role in the transcriptional regulation of PEMT expression, or (2) an association between PEMT variants and NAFLD was masked by an adequate dietary choline intake in our cohort, which was drawn from a bariatric surgery population with severe obesity in whom dietary information was not available. The gene discussed is PEMT; the disease is metabolic dysfunction-associated steatotic liver disease.