First, we analyzed the published sequence data of profiling brain cortex from transient middle cerebral artery occlusion (tMCAO) rat stroke model (data from Series GSE38037) [23], and found that SIRT1, SIRT2 and SIRT7 had no significant change, while SIRT3 significantly decreased in the brain cortex of the SD rats following tMCAO compared to the sham-operated control (Figure 2A). The gene discussed is SIRT1; the disease is Stroke.