These comprised human 518A2 melanoma, HL-60 leukemia, the multi-drug resistant carcinomas KBv1+Vbl cervix and MCF-7+Topo breast, the HT-29 colon carcinoma, the cancer stem cell-rich rat C6 astrocytoma, human U87 glioma as well as non-malignant primary mouse astrocytes and neurons, and the NE-4C neuroectodermal stem cells isolated from the fore- and midbrain vesicles of p53-deficient 9-day-old mouse embryos [14]. The gene discussed is TP53; the disease is central nervous system cancer.