Western blotting analysis showed that none of the HNSCC cells expressed CC3 protein after being treated with the ferroptosis-inducers Erastin or RSL3 (Figure 2G) as compared to N2A cells that were treated with Staurosporine (STS), an inhibitor of protein kinase C (PKC) and an inducer of apoptosis. The gene discussed is PRRT2; the disease is head and neck squamous cell carcinoma.