Tumor markers such as alpha-fetoprotein-Centaur (AFP), lectin-3-reactive alpha-fetoprotein (AFP-L3), and des-gamma-carboxyprothrombin (DCP) also showed a slight increase in NASH; however, this increase was below the established cutoff values for tumor diagnosis in NASH, but highly increased in HCC. This evidence concerns the gene ACE and metabolic dysfunction-associated steatohepatitis.