Furthermore, this is the first study to detect the cross-reacted serovars O3:K6 and K59 carrying pandemic genes (e.g., tdh, toxRS/new, and orf8), guaranteeing continuous monitoring of V. parahaemolyticus strains and allowing public health authorities to provide the best education for emergency care physicians and general practitioners in the event of local or multistate foodborne outbreaks of V. parahaemolyticus-associated gastroenteritis. Here, TDH is linked to gastroenteritis.