To verify whether LHX2 executes the oncogenic functions in ESCC progression, firstly, we built the stable LHX2-knockdown KYSE30/KYSE510 cells with high endogenous levels of LHX2 expression and LHX2-overexpressing mEC25/HNM007 cells with low basal levels of LHX2 expression. This evidence concerns the gene LHX2 and esophageal squamous cell carcinoma.