LHX2 and esophageal squamous cell carcinoma: Next, we explored the effect of LHX2 on the proliferation of ESCC cells by CCK-8 and colony formation assays, and discovered that the knockdown of LHX2 noticeably attenuated the proliferation and colony formation of KYSE30 and KYSE510 cells (Figure 2A–D), while the overexpression of LHX2 significantly promoted the proliferation and colony formation of mEC25 and HNM007 cells (Figure 2E–H).