To explain how LHX2 enhances the malignant phenotype of ESCC, we performed transcriptome profiling using LHX2-knockdown KYSE30/KYSE510 cells and their corresponding control cells, and we identified that 71 genes were upregulated and 103 genes were downregulated in both KYSE30 and KYSE510 cells after LHX2 silencing (Figure 4A,B; Supplementary Tables S1 and S2). The gene discussed is LHX2; the disease is esophageal squamous cell carcinoma.