To increase the clinical correlation of in vitro work, cytotoxicity of gemcitabine (the cornerstone chemotherapy for pancreatic cancer) was examined in the panel of pancreatic cancer cell lines in two– (traditional attached, as described above) and three-dimensional (using low attachment plates and media supplemented with B27, EGF and bFGF) morphologies. This evidence concerns the gene FGF2 and familial pancreatic carcinoma.