The isobaric tags for relative and absolute quantitation (iTRAQ) quantitative proteomics analysis of CCA cell lines (TFK1 and HuCCT1) and normal bile duct epithelial cell lines (HiBECs) secreted proteins and validation in tissues and bile and revealed that bile TPD52 and DNAJB1 are potential markers for the diagnosis and predicting prognosis of CCA; nevertheless, the sample size was too little to verify [138]. The gene discussed is DNAJB1; the disease is cholangiocarcinoma.