Wisteria floribunda agglutinin (WFA) is a probe that can specifically identify anti-sialylated mucin 1 (MUC1), and the levels of MUC1 in bile captured by WAF differ greatly between CCA patients and patients with benign biliary disease, so MUC1 in bile is promising for diagnosing CCA, but its specificity is low. Here, MUC1 is linked to cholangiocarcinoma.