TLR9 and melanoma: To overcome this challenge, a sonodynamic therapy nanovaccine integration platform fabricated by binding CpG adjuvant (TLR9 agonist) with manganese porphyrin-based metal-organic frameworks (Mn-MOF) coated by OVA-overexpressing melanoma B16 cells termed cMn-MOF@CM was designed for potentiating anti-PD-1 antibodies in the treatment of malignant melanoma.