PDX1 and neoplasm: Loss of SHH expression in endogenous PDAC cells of Pdx1-Cre; KrasLSL-G12D/+; p53fl/fl; Rosa26LSL-YFP/+; Shhfl/fl mice led to worse survival, accelerated tumor growth, a more undifferentiated tumor histology with elevated expression of epithelial to mesenchymal transition (EMT) markers (ZEB1, SLUG), and it increased metastasis compared to the corresponding ShhWT mice [75].