GLI1 was found to be overexpressed in Ewing Sarcoma cell lines, regulated by EWS-FLI1 fusion protein independent of canonical Hh signaling pathway, as evidenced by lack of GLI1 response to exogenous SHH and cyclopamine treatment, and critical for Ewing Sarcoma proliferation [198,199]. This evidence concerns the gene SHH and Ewing sarcoma.