Furthermore, NOX1 was also upregulated in the liver of NASH patients and mice fed a high-fat and high-cholesterol (HFC) diet for 8 weeks, while mice deficient in NOX1 displayed decreased levels of serum alanine aminotransferase (ALT) and hepatic cleaved caspase-3 compared to wild-type littermates when fed the HFC diet [80]. Here, NOX1 is linked to metabolic dysfunction-associated steatohepatitis.