MAP1LC3A and retinoblastoma: To quantify the autophagy activity associated with RB, GW4869 and PDT protocol, the measurement of the autophagy flux was performed by flow cytometry using a Guava Autophagy LC3 Antibody-Based Detection Kit in T98G cells in standard growing (DMEM), in HBSS starvation alone (30 min), after RB (0.75 μM) and GW (5 μM) HBSS starvation, in the absence or presence of PDT stimulation (2 min at 562/576 nm).