However, this study had certain limitations, in particular, the use of ultrasonography as the NAFLD diagnosis method, without confirmation by hepatic biopsy, and the measurement of visfatin levels in all samples (basal and 10-years after) at the end of the study, which likely affects the visfatin concentrations due to different storage times. The gene discussed is NAMPT; the disease is metabolic dysfunction-associated steatotic liver disease.