The cytokine release of IL-4, IL-10, and IFN-γ was then determined via quantitative equine capture ELISA (R&D systems, Minneapolis, MN, USA) in order to detect an allergy-mediated Th2 immune response (IL-4) and/or a proinflammatory Th1 response (IFN-γ). This evidence concerns the gene IL4 and allergic disease.