TNF and cancer: fGSEA analysis showed that multiple hallmarks were positively enriched in G361-shCYGB, including cell cycle-related hallmarks mitotic spindle, G2M checkpoint and E2F targets, as well as cancer-associated hallmarks hedgehog signaling, IL2-STAT5 signaling, inflammatory response, estrogen response early, PI3K-AKT-mTOR, KRAS signaling, TNFα signaling via NF-κB and epithelial–mesenchymal transition.