The RT-qPCR analysis of paired human bladder tissues (n = 26; 5 low grade and 21 high grade) showed that the mean ΔΔCt was 1.3454 ± 0.3784 when using β-actin as the internal control (Figure 1B) and 2.0738 ± 0.6027 when using 18S as the internal control (Figure 1C) in normal and bladder cancer tissues, indicating that normal bladder tissues contained significantly higher MT2A mRNA levels (p = 0.0014 and p = 0.002; when using β-actin or 18S as the internal control, respectively). This evidence concerns the gene ACTB and urinary bladder carcinoma.