Our results show that TLR-4, NLRP3, and inducible nitric oxide synthase (iNOS) expression was markedly increased within the mucosa following DNBS-induced colitis compared to the vehicle (31.38 ± 1.169 RFU, 14.54 ± 1.282 RFU, and 33.93 ± 1.665 RFU for TLR-4, NLRP3 and iNOS, respectively; p < 0.0001 for all comparisons; Figure 3A–D). This evidence concerns the gene NLRP3 and colitis.