OCLN and colitis: DNBS-treated mice maintained a significant impairment of colonic mucosal integrity on day 7 following colitis induction, as demonstrated by the decreased expression of the two tight junction proteins, zonula occludens-1 (ZO-1) and occludin, compared to the vehicle group (25.08 ± 3.196 FI%, p < 0.0001 for ZO-1 and 18.23 ± 1.322 FI%, p < 0.0001 for occludin; Figure 2A–C).