LGR4 and neoplasm: Finally, protein expression of LGR4 in the tissue samples of COAD was analyzed using the HPA database, as given in Figure 1D. Compared to normal tissue (Figure 1D, left panel), LGR4-positive regions in the COAD tumor tissues by immunohistochemistry (IHC) method are significantly lower than normal colon tissues, consistent with mRNA result.