Giving rats a long-term implanted gastric catheter and adding iron carbonyl (0.12% w/v in the first week and 0.25% w/v after the second week) to a high-fat diet (25% of total calories) combined with an ethanol (49% of total calories) diet, after 16 weeks of feeding, the serum levels of ALT and AST in rats rose to 2–3 times those of rats fed a normal diet, resulting in moderate to severe fatty liver as well as focal lobular central necrosis and inflammation, with some animals developing liver fibrosis and even cirrhosis [118]. Here, GPT is linked to Hepatic steatosis.