In our study, to further elucidate the regulatory effect of HSC70 on Smad3 and thoroughly investigate its relationship with TGF-β1, we used the recombinant protein TGF-β1 to stimulate ESCC cells together with DJ-1 while transfecting cells with HSC70 siRNA. The gene discussed is SMAD3; the disease is esophageal squamous cell carcinoma.