TARDBP and amyotrophic lateral sclerosis: A reduction in the proportion of motor neurons possessing nuclear TDP-43 was strongly correlated with a higher proportion of motor neurons exhibiting cytoplasmic pTDP-43 pathology (Fig. 2), reinforcing existing data demonstrating a close interdependence between TDP-43 phosphorylation, mislocalization and aggregation in spinal cord motor neurons in ALS [34].