To this end, TILs isolated from untreated melanomas were cultured with 100 U/mL IL-2, ±1 μM Ruxo (a concentration with potent suppression of p-STAT1/3 in vitro) for 3 days and then analyzed for FoxP3 expression (Fig. 6f) and production of IFN-γ/TNF (Fig. 6g). This evidence concerns the gene TNF and melanoma.