In this study, to circumvent the partial attenuation of IFN-γ signaling in IFNγR1KD melanoma and to unequivocally evaluate how tumor IFN-γ signaling affects TILs, we generate the B16 melanoma model with Ifngr1 knocked out by CRISPR-Cas9 (hereafter, IFNγR1KO). The gene discussed is IFNG; the disease is melanoma.