TNF and neoplasm: Moreover, Ruxo increased TNF, IFN-γ, perforin, and IL-2 production by CD4+ TILs (Fig. 6e), essential effector molecules in anti-tumor immunity; similar increases of IFN-γ (Supplementary Fig. 6f), perforin (Supplementary Fig. 6g), and GzmB (Supplementary Fig. 6h) were also noticed in CD8+ TILs.