AFP and hepatocellular carcinoma: Untreated or chronically undertreated FAH−/− adult pigs (No. 266, prior experiment, Fig. 2a) develop cirrhosis, adenomas, and ultimately HCC at 12 months, as shown here with hepatocellular morphological derangement, extensive stage 4 fibrosis, and significant AFP staining (Fig. 3c, right column).