SPP1 and lung cancer: To deeply dissect the role of each Mφ subcluster in the development of lung cancer, we interrogated the functions of these cell types by the single sample Gene Set Enrichment Analysis (ssGSEA) score based on the signatures of M1, M2, angiogenesis and phagocytosis.48 The LUAD enriched subclusters FABP4-Mφ and FCN1-Mφ were closely associated with phagocytosis, which is important in immune responses, while the dominant cluster in LUSC, SPP1-Mφ exhibited an enrichment of genes related to angiogenesis.