The knockdown of the Magel2 gene exclusively in ARCPOMC neurons innervating the MeA protected against DIO in both male and female mice, consistent with prior studies with PWS mouse models, including mice lacking the Magel2 and Snord116 genes (Bischof et al, 2007; Qi et al, 2016). This evidence concerns the gene SNORD116 and Prader-Willi syndrome.