For the downregulated genes in Ad-SGE-REIC-treated U87ΔEGFR glioma brain tissue compared with the control, 24 significantly enriched pathways were identified, including the TNF-alpha NF-kB Signaling Pathway, Mitochondrial Gene Expression, and Wnt Signaling Pathway NetPath (Table 2). Here, NFKB1 is linked to central nervous system cancer.