For the downregulated genes in the combination treated tissue compared with the Ad-SGE-REIC-treated U87ΔEGFR glioma tissue, seven significantly enriched pathways were identified, including the Robo4 and VEGF Signaling Pathways Crosstalk and TNF-alpha NF-kB Signaling Pathway (Table 4). This evidence concerns the gene TNF and central nervous system cancer.