The diagnostic accuracy of LC-SPIK, AFP, and PIVKA-II for the detection of HCC was 0.841 (95% CI 0.763–0.901), 0.719 (95% CI 0.630–0.797), and 0.853 (95% CI 0.777–0.911), respectively (Figure 2A); no significant difference was observed between the performance of LC-SPIK and PIVKA-II (DeLong test: p = 0.809). This evidence concerns the gene AFP and hepatocellular carcinoma.