Figure 4 shows the duplicate blots of the expressed proteases, paired with the positive reference, a negative and the undetected MMP-3. Three proteases were reliably detected; the aspartic endo-protease- Cathepsin D; the lysosomal cysteine- Cathepsin B; urokinase plasminogen activator receptor- uPAR (Figure 4, Table 4). Unsurprisingly, both Cathepsin D and Cathepsin B are associated with malignant melanoma progression in human and mouse models [41,43]. The gene discussed is CTSD; the disease is melanoma.