The expression of the identified Irf1 target genes CFL4, CFL5 and FTR1 (Figure 2B), which are involved in the acquisition of iron by the reductive pathway, were also upregulated by iron deficiency and by the overexpression of IRF1, which agrees with our RNA-Seq data and the published data of the iron deficiency transcriptome (Lan et al., 2004). Here, IRF1 is linked to nutritional disorder.