IRF1 and Iron deficiency anemia: At the later time point of IRF1 overexpression (4 hours), 72 genes coincided, but in contrast to the early time point, some genes showed opposite regulation, 29 genes activated by IRF1 were repressed under iron deficiency, and 23 genes inhibited by IRF1 were activated under iron deficiency (Figure 4A), only 18 genes were equivalently activated, and 2 genes were repressed.