However, there is a gradual decrease in the SPISE index (which is an indicator of whole-body insulin sensitivity and calculated based on TG, high-density lipoprotein (HDL), and BMI values) from healthy to prediabetes and newly diagnosed T2D cases. SPISE index and HOMA-B values were found significantly (p=0.001 and p<0.001) decreased only in the newly diagnosed T2D group compared to the healthy control group. The gene discussed is INS; the disease is prediabetes syndrome.