By analysis of microarray datasets and in vitro confirmative experiments, we demonstrated that the NAFLD (including IL-6, BID, and PRKAA2 genes) and HIF-1 signaling pathways (including IL-6, HIF-1, and HMOX1 genes) were ferroptosis-related pathways in CAVD. Here, HMOX1 is linked to congenital bilateral aplasia of vas deferens from CFTR mutation.