To investigate the potential clinical efficacy of PTS in immunotherapy, we examined the distribution of checkpoint-related genes (LAG3, HAVCR2, PDCD1LG1, IDO1, TIGIT, PDCD1, PD-L1, and CTLA-4) and tumor mutation burden (TMB) in different PTS subgroups and found that LAG3, PDCD1LG1, IDO1, TIGIT, PDCD1, PD-L1, and CTLA-4 were upregulated in patients with low risk in the TCGA training set (Supplementary Figure S4A), while TMB was higher in patients with high risk (Supplementary Figure S4B). The gene discussed is HAVCR2; the disease is neoplasm.