APC and colorectal carcinoma: We next estimated the general cytotoxicity of MAIT-516 in normal intestinal epithelial cells (HIEC-6) and CRC cells expressing full-length (RKO, LS-513, LS-1034, NCI-H508, NCI-H716, and SNU-C2B) or truncated (Caco-2, HT-29, LoVo, DLD-1, SW480, and SW620) APC using the CCK-8 assay16,27.