As the W593A and N641A mutants significantly reduced the binding affinity of MAI-516 to truncated APC, to inspect the biological relevance of these mutants in CRC cells, we constructed SW480 stable cells overexpressing truncated APC (1–1338, the same as the endogenous APC expressed in SW480 cells) and the W593A and N641A mutants (Supplementary Fig. 14a). The gene discussed is APC; the disease is colorectal carcinoma.