Comprehensive immune characterizations revealed that the mechanical stress deriving from HIFU could promote ICD of human breast cancer cells through tumor necrosis‐associated signal pathways, which significantly increased the damages‐related molecular patterns (e.g., CRT, HSP70, and HMGB‐1) and inflammatory cytokines (IFN‐γ, IL‐1α, IL‐1β, and IL‐18), and drove the secretion of chemokine (IL‐8) relating to M1 macrophage activation.[131]. The gene discussed is IL1B; the disease is breast cancer.