To test the effects of CaMK4 inhibition and AMPK activation on in vitro cyst formation, ADPKD cells were seeded within a collagen matrix and stimulated to form cysts with the treatment of epidermal growth factor (EGF) and forskolin (FSK), an activator of adenylyl cyclase. The gene discussed is EGF; the disease is autosomal dominant polycystic kidney disease.