To identify that miR-488-5p inhibited liver fibrosis by targeting TET3 which was related with TGF-β/SMAD2/3 pathway, first, we evaluated the α-SMA expression of liver tissues through IHC staining; our data showed that α-SMA expression was remarkably inhibited in the liver tissues from CCl4, HFD and BDL group which were transfected with ago-miR-488-5p (Fig. 7a, b). This evidence concerns the gene SMAD2 and Hepatic fibrosis.