SH2D3A and infection: To investigate whether infection with WT or NSP1 mutant rotavirus induces a type I or type III IFN-mediated antiviral response, WT pMx1-mCherry and IFNLR1−/− pMx1-mCherry cells were infected at an MOI of 0.1 and UnaG (rotavirus)- and mCherry (ISG)-positive cells were quantified over time.