In a murine in vivo NAFLD model glucose (GLU), total cholesterol (TC) triacylglycerol (TG) as well as glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) and gamma-glutamyl transferase (GGT) serum concentrations were all significantly reduced (P < 0.001) and the expression of nuclear factor-κB (NF‐κB) was significantly decreased in DSSG treated compared to untreated NAFLD animals (P < 0.001). Here, NFKB1 is linked to metabolic dysfunction-associated steatotic liver disease.