To explore changes in the DNA methylome and transcriptome during the development of acquired resistance to an ALK inhibitor in NSCLC, we performed 450 K BeadChip DNA methylation analysis and scRNA-seq of the EML4-ALK fusion-positive lung cancer cell line H3122 and the ceritinib-resistant cell line LR established (Fig. 1a, b). Here, ALK is linked to non-small cell lung carcinoma.