To analyze the in vivo efficacy of FLT3 inhibition combined with autophagy inhibition, we generated an orthotopic AML xenograft model by tail vein injection of luciferase-labeled human MV4-11 cells into NSG (NOD/severe combined immunodeficient (SCID)/Il2rg−/−) mice (Fig. 5A). Here, FLT3 is linked to acute myeloid leukemia.