All three FLT3i induced autophagy in a concentration-dependent manner in cells expressing FLT3-ITD (MV4-11, MOLM-14, 32D-FLT3-ITD), whereas cells expressing wildtype FLT3 (HEL-276, HL-60, OCI-AML-3, THP-1) or no FLT3 (32D-ctrl) did not show any response (Fig. 3B). This evidence concerns the gene FLT3 and acute myeloid leukemia.