As definitive proof, the stable cloning of the putative ERα-LBD CDS into BC cells (exon 4 to exon 8), led to ERα-LBD overexpression (oe) whereas targeted genomic deletions within exon 4 (by CRISPR technology) induced ERα-LBD knockdown (kd) (Supplementary Fig. 2d). The gene discussed is ESR1; the disease is breast cancer.