To assess whether local injection of PIC into an RT-treated tumor could improve the systemic anti-tumor immune response when given in conjunction with anti-CTLA-4, we generated mice bearing two B78 melanoma tumors, one on the right flank and the other one implanted 7 days later on the left flank (to simulate a smaller distant site of metastasis). The gene discussed is CTLA4; the disease is neoplasm.