We observed that PIC + RT + anti-CTLA-4 treatment significantly reduced the progression of these left flank tumors compared to all other treatment groups and the triple treatment combination (PIC + RT + anti-CTLA-4) rendered 4/9 mice completely disease-free; no mice in any other group became tumor-free (Fig. 7b–d). The gene discussed is CTLA4; the disease is neoplasm.