ADAM17 and malignant colon neoplasm: For that evaluation, we cocultured PMA-differentiated THP-1 cells with MDA-MB-231 (MDA-231) human breast cancer cells or SW480 human colon cancer cells, treated with either negative control (NC) or ADAM17 siRNA for 48 hours (Figure 3F), and evaluated the macrophage potential to induce cancer cell invasion (Figure 3B).