ZIC4 and multiple system atrophy: Quantification of the proportions of ZIC4-positive neurons among the total number of dentate nucleus neurons depicted relatively constant proportions in healthy controls and patients with MSA-SND (33.2% ± 0.0% vs 32.6% 0.0%), whereas in patients with MSA-OPCA or MSA-mixed phenotype, we found significantly lower percentages of ZIC4-positive neurons (15.5% ± 0.1%) (Fig. 3J).