CARD8 and lung adenocarcinoma: To elucidate the role of CARD8-AS1 in lung adenocarcinoma, the pcDNA3.1-CARD8-AS1 plasmid was transfected into A549 and PC9 cells and the results as shown in Figure 2B suggested that CARD8-AS1 expression increased dramatically in transfected cells with pcDNA3.1-CARD8-AS1 (p < 0.001).