CD4 and neoplasm: As Treg depletion may enhance anti-vaccinia cellular immunity in tumour-bearing mice (Figures 2(b) and 2(c)), the potential role of JTT in reducing the number of Tregs was investigated by comparing the number of CD4+ CD25+ Foxp3+ Tregs in the spleens derived from tumour-bearing mice fed with a control or JTT diet for a total of 21 days (7 dpi with vSC25).